(Flow Cytometry, FCM) is sero reCellerzer ayo anoyera iyo fluorescence huwandu hwemakona akaomeswa. Iyo ndiyo yepamusoro-techney tekinoroji yakagadzirwa zvichienderana nekuongororwa uye kuronga kwemasero asina kuroora. Zvinogona kukurumidza kuyera uye kutsaura kukura, chimiro chemukati, DNA, rna, mapuroteni, zvidzitiro uye zvimwe zvemuviri kana zvemakemikari zvemakemikari, uye zvinogona kuve zvakavakirwa pakuunganidzwa kwezvinhu izvi.
Kuyerera Cytometer kunyanya kune zvikamu zvishanu zvinotevera:
1 kuyerera chammer uye fludics system
2 Laser Chiedza Tsime uye Beam Sping System
3 Optical system
4 zvemagetsi, kuchengetedza, kuratidzwa uye kuongororwa system
5 sero rekugadzirisa system
Pakati pavo, Laser anowedzera muLaser Chiedza sosi uye danda kugadzira system ndiko kuyerwa kukuru kwekufungidzira kwemasaini mune inoyerera cytometry. Kukura kwechiedza chakajeka uye nguva yekufumurwa ine hukama nehukuru hwechiratidzo cheFluorescence. Laser ndeye colent cleance sosi inogona kupa imwe-wavelength, yakakwirira-yakakura, uye yakakwirira-yekukanganisa kuvhenekerwa. Iyo ndiyo yakanaka yekubvumidza inofadza sosi yekusangana izvi zvinodiwa.
Kune maviri cylindrical lenses pakati peiyo laser sosi uye iyo inoyerera chivakwa. Iyi lenses inotarisa laser beam ine circular muchinjiko-chikamu chakaburitswa kubva kune laser sosi mune elliptic nguwo ine diki cross-chikamu (22 μM × 66 μm). Iyo laser simba mukati meiyi elliptical danda rinoparadzaniswa maererano nekuparadzira kwakajairika, kuve nechokwadi chekuvhenekera kwekuwedzera kwemasero kupfuura iyo nzvimbo inopfuura neye laser yekuchinjika nzvimbo. Kune rimwe divi, iyo system yeOptical ine akawanda maseti emasendimita, mapipi, uye mafirita, ayo anogona kukamurwa kuita mapoka maviri: kumusoro uye kudzora kwekamuri yekuyerera.
Iyo Optical system pamberi pekamuri inoyerera ine lens uye pinile. Basa guru re lens uye pini Izvi zvinogovera iyo laser simba zvinoenderana nekuparadzira kwakajairika, kunoita kuti uve nechokwadi chekuvhenekera kwemasero kupfuura iyo laser yekuona nzvimbo uye kudzikisira kupindira kubva kuchiedza chisina kujeka.
Kune matatu matatu makuru emhando dzemafirita:
1: Long Pass Filter (LPF) - Ingotendera mwenje ne wavelengths yakakwira kupfuura kukosha kwakananga kupfuudza.
2: Pfupi-Pass Filter (SPF) - Ingotendera mwenje ne wavelengths pazasi kukosha kwakananga kupfuura.
3: BandPass Filter (BPF) - Inongobvumira mwenje mune yakatarwa wavelength renji kuti ipfuure nepakati.
Kusanganiswa kwakasiyana kwefirivheri kunogona kuendesa madhiri emasaini pane akasiyana wavelengths kusvika pamunhupoto wemifananidzo (pmsts). Semuenzaniso, mafirita ekuona girini fluorescence (Fitc) pamberi pePMT are lpf550 uye BPF525. Mafirita anoshandiswa kuona orange-red fluorescence (pe) pamberi pePMT are lpf600 uye BPF575. Mafirita ekuona kutsvuka tsvuku fluorescence (CY5) pamberi pePMT ndeye lpf650 uye BPF675.
Kuyerera Cytometry kunonyanya kushandiswa kurongedza cell. Nekufambira mberi kwehunyanzvi hwekombuta, kuvandudzwa kwemachira uye kugadzirwa kweMonoclonal Anti Izvi zvekushandisa zvinosanganisira cell dynamics kuongororwa, cell Apoptosis, cell typing, tumor kuongororwa, zvinodhaka zvinodhaka kuongororwa, nezvimwe.
Kutumira Nguva: Sep-21-2023
