(Flow cytometry, FCM) is a cell analyzer inoyera fluorescence intensity ye stained cell markers. Iyo tekinoroji yepamusoro-soro yakagadziridzwa yakavakirwa pakuongorora uye kuronga kwesero rimwe chete. Inogona kukurumidza kuyera uye kuronga saizi, chimiro chemukati, DNA, RNA, mapuroteni, maantigen uye zvimwe zvemuviri kana kemikari zvimiro zvemasero, uye zvinogona kubva pakuunganidzwa kwezvikamu izvi.
Flow cytometer inonyanya kuve nezvikamu zvishanu zvinotevera:
1 Kuyerera kamuri uye fluidics system
2 Laser mwenje sosi uye danda kuumba system
3 Optical system
4 Electronics, kuchengetedza, kuratidza uye kuongorora system
5 Masero ekuronga hurongwa
Pakati pavo, laser excitation mune laser mwenje sosi uye danda rekugadzira sisitimu ndiyo chiyero chikuru chefluorescence masaini mukuyerera cytometry. Kusimba kwechiedza chekusimudzira uye nguva yekuratidzira yakabatana nekusimba kwechiratidzo chefluorescence. Laser ndeye yakabatana mwenje sosi iyo inogona kupa imwechete-wavelength, yakakwirira-yakanyanya, uye yakakwirira-kugadzikana kuvhenekera. Ndiyo yakanaka excitation chiedza sosi kusangana nezvinodiwa izvi.
Kune maviri cylindrical lenses pakati peiyo laser sosi uye yekuyerera kamuri. Aya malenzi anotarisa laser danda rine denderedzwa muchinjiko-chikamu chinoburitswa kubva kune laser sosi kuita elliptical danda rine diki-chikamu-chikamu (22 μm × 66 μm). Iyo laser simba mukati meiyi elliptical danda inogovaniswa zvinoenderana neyakajairwa kugovera, kuve nechokwadi chisingachinji chekuvhenekera kusimba kwemaseru anopfuura nepanzvimbo yekuona laser. Kune rimwe divi, iyo optical system ine akawanda seti emalenzi, pinholes, uye mafirita, ayo anogona kukamurwa kuita mapoka maviri: kumusoro uye kuzasi kwekamuri yekuyerera.
Iyo optical system pamberi pekamuri yekuyerera ine lens uye pinhole. Basa guru relenzi uye pinhole (kazhinji malenzi maviri nepinhole) nderekutarisisa danda relaser rine denderedzwa remuchinjiko-chikamu chinoburitswa neiyo laser sosi mune elliptical danda rine diki-chikamu. Izvi zvinogovera simba relaser zvinoenderana nekugoverwa kwakajairwa, kuve nechokwadi chekusimba kwekuvhenekera kwemaseru munzvimbo yekuona laser uye kuderedza kukanganiswa kubva kuchiedza chakarasika.
Kune marudzi matatu makuru emasefa:
1: Yakareba pfuura sefa (LPF) - inongobvumira mwenje une mafungu akareba kupfuura kukosha kwakati kuti upfuure.
2: Short-pass sefa (SPF) - inongobvumira mwenje une wavelengths pazasi pemutengo chaiwo kuti upfuure.
3: Bandpass sefa (BPF) - inongobvumira mwenje mune yakatarwa wavelength renji kuti ipfuure.
Musanganiswa wakasiyana wemafirita unogona kutungamira masaini efluorescence pamawavelengths akasiyana kune ega photomultiplier chubhu (PMTs). Semuenzaniso, mafirita ekuona green fluorescence (FITC) pamberi pe PMT iLPF550 neBPF525. Masefa anoshandiswa kuona orenji-tsvuku fluorescence (PE) pamberi pe PMT iLPF600 uye BPF575. Masefa ekuona dzvuku fluorescence (CY5) pamberi pe PMT iLPF650 uye BPF675.
Flow cytometry inonyanya kushandiswa kugadzirisa masero. Nekufambira mberi kwehunyanzvi hwekombuta, kuvandudzwa kweimmunology uye kugadzirwa kwemonoclonal antibody tekinoroji, mashandisirwo ayo mubiology, mushonga, pharmacy uye mamwe minda ari kuwedzera kupararira. Zvishandiso izvi zvinosanganisira kuongororwa kwesero dynamics, cell apoptosis, cell typing, tumor kuongororwa, kuongororwa kwezvinodhaka, nezvimwe.
Nguva yekutumira: Sep-21-2023
